Appearance (Colour)
Light biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Sterility (Bacterial count)
No growth is observed after 14 days at 30-35°C incubation for bacteria and at 20-25°C for fungi
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-48 hours for bacteria or upto 7 days for fungi *
Organism
Candida albicans * ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 20 - 25°C
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Micrococcus luteus ATCC 9341
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Incubation temperature : 35 ± 2°C
Applications
A nutritious general purpose medium, prepared by replacing animal based peptones with vegetable based peptones. It is gamma irradiated sterile powder, used in evaluation of sterility in manufacturing process.
Composition
Ingredients
gm/lt.
Veg. Hydrolysate
17.00
Papaic digest of soybeanmeal
3.00
Dipotassium phosphate
2.50
Sodium chloride
5.00
Dextrose
2.50
Directions
1. Add 30.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Do not autoclave.
Note : Sterile powder can be used directly.
