Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.60 gm/liter
Solubility before autoclaving (Clarity)
Clear w/o any precipitate
pH (25°C)
6.8 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear w/o any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light yellow
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Gas production : (+ve)
Indole production (at 44°C) : (+ve)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Gas production : (+ve)
Indole production (at 44°C) : (-ve)
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Gas production : (-ve)
Indole production (at 44°C) : (-ve)
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : Inhibited
Applications
For the detection of coliform bacteria in a variety of specimens. Also for the enumeration of coliform bacteria by the multiple tube fermentation technique.
Composition
Ingredients
gm/lt.
Tryptose
20.00
Lactose
5.00
Sodium chloride
5.00
Dipotassium phosphate
2.75
Potassium phosphate
2.75
Sodium lauryl sulphate
0.10
Directions
1. Add 35.60 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes containing inverted Durham’s tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
