Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
45.30 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate :>=50%
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate :>=50%
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103, Growth : Inhibited
Organism
Escherichia coli
ATCC 25922
Inoculum
>=103, Growth : Inhibited
Applications
For the selective isolation, cultivation and identification of Pseudomonas aeruginosa.
Composition
Ingredients
gm/lt.
Peptone
20.00
Magnesium chloride
1.40
Potassium sulphate
10.00
Cetrimide
0.30
Agar
13.60
Directions
1. Add 10 ml of glycerol to distilled/purified water.
2. Add 45.30 gm powder to distilled/purified water.
3. Bring volume to 1.0 liter and mix thoroughly.
4. Gently heat and bring to boiling.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
6. If desired,add aseptically rehydrated contents of 1 vial of Nalidixic Selective Supplement (67124) to 1.0 liter of medium.
7.Mix well and dispense.
