Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm,comparable with 1.5% gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate : >=70.0%
Organism
Escherichia coli ATCC 23724
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate : >=70.0%
Organism
Escherichia coli ATCC 33526 (K802)
Inoculum (cfu) 10-100
Growth: Good
Recovery Rate : >=70.0%
Applications
Used for maintaining and cultivating recombinant strains of Escherichia coli.
Composition
Ingredients
gm/lt.
Tryptone
10.00
Yeast extract
5.00
Sodium chloride
5.00
Agar
15.00
Directions
1. Add 35.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
