Appearance (Colour)
Light yellow to light blue
Appearance (Form)
Free flowing, homogeneous powder
Solubility
90.07 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Dark blue
Cultural Response
Inoculate and Incubate at 35 -37°C for 18 - 48 hours
Organism
Enterococcus faecalis
ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : blue-black
Organism
Streptococcus pyogenes
ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : > =50.0%
Colony colour : blue
Organism
Streptococcus salivarius
ATCC 13413
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >= 50.0%
Colony colour : blue (gum drop)
Organism
Escherichia coli
ATCC 25922
Inoculum
>=10³
Growth : Inhibited
Organism
Staphylococcus aureus
ATCC 25923
Inoculum
>=10³
Growth :Inhibited
Applications
Selective medium for the isolation of streptococci and enterococci from clinical specimens.
Composition
Ingredients
gms/lt.
Tryptone
15.00
Peptone
5.00
Dextrose
1.00
Sucrose
50.00
Dipotassium phosphate
4.00
Trypan Blue
0.075
Crystal Violet
0.0008
Agar
15.00
Directions
1. Add 90.07 gms powder to 1.0 liter distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressue at 121°C for 15 minutes.
4. Cool to 45-50°C and aseptically,add 1 ml of sterile 1% Potassium Tellurite solution (91488).
5. Mix well.
