Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.08 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony characteristics : Purple-magenta (mucoid)
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Organism
Enterobacter aerogenes ATCC 13048
Inoculum
Growth : Inhibited
Organism
Salmonella typhimurium ATCC 14028
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation and detection of Klebsiella species from water and other sources. This medium can also be used in membrane filtration procedure.
Composition
Ingredients
gm/lt.
Peptone
10.00
Yeast extract
7.00
Meat extract
2.00
Sodium chloride
5.00
Bile salts mixture
1.50
Sodium lauryl sulphate
0.10
Chromogenic mixture
0.2
Agar
15.00
Directions
1. Add 20.4 gm powder to 500.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not Autoclave.
4. Cool to 45-50°C and aseptically add the rehydrated contents of 1 vial of Klebsiella Selective Supplement (29760).
5. Mix well and pour into sterile petri plates.
