Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
45.03 gm/liter
Solubility before boiling (clarity)
Clear to slightly opalescent with no significant precipitate
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to very slightly opalescent with no significant precipitate
Prepared medium appearance after boiling (colour)
Reddish orange
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : ~50.0%
Colony colour : pink w/bile precipitate
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : ~50.0%
Colony colour : colourless
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : ~50.0%
Colony colour : colourless
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : ~50.0%
Colony colour : colourless
Organism
Enterococcus faecalis ATCC 29212
Inoculum
>=10³,
Growth : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=10³,
Growth : Inhibited
Applications
Differential medium for direct counts of coliforms from dairy products and for the isolation of enteric microorganisms from clinical and non-clinical specimens.
Composition
Ingredients
gms/lt.
Peptone
10.00
Agar
15.00
Lactose
10.00
Sodium chloride
5.00
Dipotassium phosphate
2.00
Ferric citrate
1.00
Sodium citrate
1.00
Sodium deoxycholate
1.00
Neutral Red
0.03
Directions
1. Add 45.03 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
