Appearance (Colour)
Light beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility after boiling (Clarity)
Light amber, clear without any precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance
Light amber, clear without any precipitate
Sterility (Bacterial count)
No growth is observed after 14 days at 30-35°C incubation for bacteria and at 20-25°C for fungi.
Stability (After autoclaving)
Stability test : Light amber, clear solution without any precipitation or sedimentation at room temperature for 7 days.
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-48 hours or upto 7 days
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 35 ± 2°C
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth :- Good
Incubation temperature :- 20 - 25°C
Applications
Gamma irradiated sterile powder used for the growth of wide variety of bacteria and fungi.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
17.00
Papaic digest of soybeanmeal
3.00
Dipotassium phosphate
2.50
Sodium chloride
5.00
Glucose
2.50
Directions
1. Sterile powder can be used directly.
2. For preparation of sterile liquid medium, aspetically add 30.00 gms in 1.0 liter of distilled/deionised water.
3. Gently heat to dissolve the medium.
4. Do not autoclave or overheat.
Note : If desired, filter solution through 0.22 micron filter to eliminate any possibility of presence of fibres.
