Appearance (Colour)
Light yellow to light green
Appearance (Form)
Free flowing, homogeneous powder
Solubility
22.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.54% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light purple
Cultural Response
Inoculate and incubate at 37°C ± 2°C for 18 - 24 hours.
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Recovery : =>70%
Colour : Yellow
Organism
Bacillus cereus ATCC 10876
Inoculum (cfu) 10-100
Growth : Good
Recovery : =>70%
Colour : Colourless
Applications
Used for the differentiation between Bacillus cereus and Bacillus subtilis based on mannitol fermentation.
Composition
Ingredients
gms/lt.
Mannitol
5.00
Yeast autolysate
0.20
Monohydrogen ammonium phosphate
1.00
Potassium chloride
0.20
Magnesium sulphate
0.20
Bromocresol purple
0.0075
Agar
15.40
Directions
1. Add 22.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
