Sisco Research Laboratories
Pvt. Ltd.
Product Datasheet
and Specification
Specifications
Appearance (Colour)
Yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
40.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Note: After addition of 5% sterile defribnated blood: Cherry red, opaque
Cultural Response
Inoculate and incubate at 37°C for 18- 24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70%
Haemolysis : Beta
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70%
Haemolysis : None
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=70%
Haemolysis : Beta
Applications
For the preparation of blood agar by the addition of sheep blood.
Composition
Ingredients
gm/lt.
Agar
15.00
Sodium chloride
5.00
Pancreatic digest of casein
13.00
Yeast extract
5.00
Heart muscle solids from infusion
2.00
Directions
1. Add 40.00 gm powder to distilled/purified water.
2. Bring volume to 950 ml and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45-50°C.
6. Add aseptically 50.0 ml sterile defibrinated sheep blood, Mix thoroughly.
General Information

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :

100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :

100 Gms

Plastic Bottle

Available Packages
Pack Size
100 Gms
Pack Size
500 Gms