Appearance (Colour)
Light beige
Appearance (Form)
Free, flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear w/o any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 35-37°C for 18-48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
For the culture of blood clots from patients with suspected enteric fever.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
20.00
Sodium chloride
5.00
Sodium taurocholate
5.00
Directions
1. Add 30.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 40°C and add 1 ml of Streptokinase solution 100000 units/ml).
6. Mix well and dispense as desired.
