Appearance (Colour)
Light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
63.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5 % agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opaque
Prepared Medium Appearance after autoclaving (Colour)
Yellow
Cultural Response
Inoculate and incubate at 37°C for 24-48 hours
Organism
Staphlococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth- Good, Recovery Rate : >=50.0 %,Colony color- Black, Lecithinase halo- (+)
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth- Good, Recovery Rate : >=50.0%,Colony color- Black, Lecithinase halo - (+)
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth- Good, Recovery Rate : >=50.0%,Colony color- Brown, Lecithinase halo- -
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth- Good,Recovery Rate : >=50.0%, Colony color- Black, Lecithinase halo- Nil
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth- Poor- Fair, Colony color- Black, Lecithinase halo- Nil
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth- Good, Colony color- Black, Lecithinase halo- Nil
Organism
Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100
Growh- Poor-good, Colony color- very small shades of black- brown, Lecithinase halo -Nil
Applications
Used as base for egg-tellurite-glycine agar for the selective isolation of coagulase positive staphylococci from food, skin, soil, air and other materials.
Composition
Ingredients
gm/lt.
Agar
20.00
Glycine
12.00
Sodium pyruvate
10.00
Pancreatic digest of casein
10.00
Beef extract (ex.buffalo)
5.00
Lithium chloride
5.00
Yeast extract
1.00
Directions
1. Add 63.00 gm powder to 950.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of egg yolk emulsion.Mix well and dispense.
