Appearance (Colour)
Light greenish biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.7 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 550C for 36- 48 hours
Organism
Bacillus coagulans ATCC 8038
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : > 50 - 70.0%, Colony colour : Yellow
Organism
Bacillus stearothermophilus ATCC 7953
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >50 -70.0%, Colony colour : Yellow
Organism
Bacillus brevis ATCC 8246
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >50 -70.0%, Colony colour : Yellow
Applications
For the isolation and cultivation of mesophilic and thermophilic aerobic microorganisms related to food spoilage.
Composition
Ingredients
gms/lt.
Tryptone
10.00
Dextrose
5.00
Bromocresol Purple
0.04
Agar
15.00
Directions
1. Add 30.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
