Appearance (Colour)
Yellowish green
Appearance (Form)
Free flowing, homogeneous powder
Solubility (Colour) 2% aq. solution
Yellow, clear
Clarity of solution
1% w/v aq. solution is clear and free from extraneous matter
pH (25°C)
5.5 - 7.0 (2%)
Stability (After autoclaving)
Yellow, clear without any precipitate or scum on the surface of the solution
Loss on drying
< 5.0%
Total Aerobic microbial count
(By Plate Count Method) : <= 10, 000 cfu/gm
Cultural Response
Inoculated and incubated at 37°C for 18 - 24 hours.
(MacConkey Agar prepared using Bile Salts as ingredient)
Organism
Escherichia coli ATCC 25922 (pink to red colonies with bile precipitate)
Inoculum (cfu) 10-100
Growth : Good
Organism
Salmonella typhimurium ATCC 14028 (colourless colonies)
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Inhibited
Deoxycholic acid-sodium salt
~ 50.00%
Cholic acid-sodium salt
~ 50.00%
Substance insoluble in 84% ethanol
< 0.1%
Applications
Used in microbiological culture media as a selectively inhibitory agent.
