Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
55.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light red to red
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : pink to red w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : pink to red
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : pink, mucoid
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery Rate : >=50.0%
Colony colour : colourless
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Salmonella paratyphi A
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Salmonella paratyphi B
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery Rate : >=70.0%
Colony colour : pale pink-red
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Colony colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Differential medium for detection and isolation of enteric organisms by restricting the swarming of Proteus species.
Composition
Ingredients
gms/lt.
Peptic digest of animal tissue
20.00
Agar
20.00
Lactose
10.00
Sodium taurocholate
5.00
Neutral Red
0.04
Directions
1. Add 55.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
