Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
18.12 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light yellow
Cultural Response
Inoculate and incubate at 35°C for 18- 24 hours or upto 7 days
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Salmonella serotype Typhi ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Salmonella serotype Enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Applications
Used for enumerating heterotrophic bacteria from drinking water.
Composition
Ingredients
gm/lt.
Casein acid hydrolysate
0.50
Yeast extract
0.50
Proteose peptone
0.50
Dextrose
0.50
Starch, soluble
0.50
Dipotassium phosphate
0.30
Magnesium sulphate
0.024
Sodium pyruvate
0.30
Agar
15.00
Directions
1. Add 18.12 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
