Agarose gel electrophoresis
Extracted DNAs molecular size is compared with High Range DNA marker
Stability
Stable for one year giving optimal yield if stored as instructued
Storage
Store all reagents at Room Temperature
Description
Genomic DNA Extraction Mini spin column kit is a research kit used for isolation of genomic DNA. The kit combines the selective binding properties of a silica gel- based membrane, with the speed of micro spin. The sample is lysed under highly denaturing conditions, to ensure release of intact DNA. Buffering conditions are then adjusted to provide optimum binding of the DNA to the column and the sample is loaded on to a spin column. The DNA binds to the membrane and contaminants are efficiently washed away using two different wash buffers. High quality DNA is eluted in a special buffer, ready for direct usage and storage.
Includes
| Components | 25 Extractions | 50 Extractions |
|---|
| Spin Column with collection tubes | 25 Nos. | 50 Nos. |
| Collection tubes (2ml) | 50 Nos. | 100 Nos. |
| Micro-Centrifuge tubes (1.5ml) | 25 Nos. | 50 Nos. |
| Binding Buffer | 6.0 ml | 12.0 ml |
| Reconstitution Buffer | 6.0 ml | 12.0 ml |
| Wash Buffer 1 (Concentrate) | 10.0 ml | 20.0 ml |
| Wash Buffer 2 (Concentrate) | 6.25 ml | 12.5 ml |
| Elution Buffer | 5.0 ml | 10.0 ml |
| Proteinase K (lyophilized) | 12.5 mg | 25 mg |
| Proteinase K Dilution Buffer | 0.625ml | 1.25 ml |
| Precipitation Buffer | 3.0 ml | 6.0 ml |
