Appearance (Colour)
Yellow to brown
Appearance (Form)
Free flowing, homogeneous powder
Solubility
10% aq. solution is medium amber, clear, completely soluble
pH (2% aq. solution)
6.50 - 7.20
Stability (After autoclaving)
Medium amber to yellowish brown, clear
Suitability for Cell Culture
Passes Test
Total Aerobic microbial count
<= 10,000.0 cfu/gm
Total Yeast and Mould count
<= 100.0 cfu/gm
Nitrogen (N) content
<= 10.00 - 12.00 %
Ash
11.0 - 16.0%
Loss on drying
<= 5.0%
NaCl
<= 5.0%
Coagulable Protein Test
Passes Test
Cultural Response
Inoculate and incubate at 35-37°C for 18-24 hours with the following
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Indole production : Positive
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Escherichia coli
Test for presence of Escherichia coli :Absent
Salmonella/25gm
Test for presence of Salmonella /25 gm : Absent
Applications
Water soluble portion of autolysed yeast, suitable for cell culture applications. Many media containing Yeast extract have been recommended for use in fermentations, cell culture media, molecular genetic media, and culture media for cultivation and resuscitation of a large variety of fastidious microorganisms and antibiotics assays.
