Appearance (Colour)
Light biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
36.55 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 44°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony Colour : Blue- green
Organism
Klebsiella Pneumoniae ATCC 29665
Inoculum (cfu) 10-100
Growth : Poor - fair
Colony Colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : Inhibited
Applications
A selective chromogenic medium used for the detection and enumeration of Escherichia coli in food samples,animal feeds and water.
Composition
Ingredients
gm/lt.
Peptone
20.00
Bile salts
1.50
X-Glucuronide
0.075
Agar
15.00
Directions
1. Add 36.57 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
