Appearance (Colour)
White
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 1% aq. solution
Clear
Solubility (Colour) 1% aq. solution
Colourless
Activity
min 0.2 Anson units/g
min 50 BAEE units/mg
Suitability for Cell Culture
Passes
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg)
<0.05 EU/mg
Unit Definition
One BAEE unit of Trypsin activity will produce ΔA at 253 nm of 0.001 per minute with BAEE as substrate at pH 8.0 at 25 °C in a reaction volume of 3.0 mL.
3 BAEE units = 1 NF unit
Enzyme solution for activity determination
Prepared in 0.001 N Tris HCl.
Notes
1 Anson unit is amount of enzyme which under standard conditions of Anson hydrolyzes Haemoglobin at such an initial rate the amount of hydrolysis product formed per min has the same extinction on reaction with phenol reagent as 1 mmole of tyrosine Ref M.L.Anson J.gen Physiol 22,79,1939 & 17,151,1933
