Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing,homogeneous powder
Solubility
45.70 gms/lit.
pH (25°C)
7.1 - 7.5
Solubility before autoclaving (Colour)
Light to medium amber
Solubility before autoclaving (Clarity)
Clear to opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent,may have slight precipitate
Gel strength
Firm,comparable with 1.5% Agar gel
Cultural Response
Inoculate and incubate at 35-37°C for 18-24 hours for bacteria and at 25 °C for fungal * organisms for upto 3-5 days.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Micrococcus luteus ATCC 9341
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Aspergillus niger * ATCC 16404
Inoculum (cfu) 10-100
Growth : Good
Organism
Candida albicans * ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Applications
Tryptone Soya Agar w/ Lecithin and Polysorbate 80 is used for validation of cleanliness on surfaces of containers,equipment surfaces and water miscible cosmetics.
Composition
Ingredients
gms/lt.
Enzymatic digest of casein
15.0
Papaic digest of soyabean meal
5.0
Sodium chloride
5.0
Polysorbate 80 (Tween 80)
5.0
Lecithin
0.7
Agar
15.0
Directions
1. Add 45.70 gm powder to 1.0 litre distilled /purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes
