Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
18.00 gm/liter
Solubility before autoclaving (Clarity)
Clear solution
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution
Prepared Medium Appearance after autoclaving (Colour)
Light yellow coloured
Cultural Response
Inoculate and incubate at 350C - 370C for 24-48 hours
Organism
Candida albicans ATCC 10231
Inoculum
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Good
Applications
Used as diluting or rinsing fluid, when performing. sterility testing of pharmaceuticals.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
5.00
Beef extract
3.00
Polysorbate 80
10.00
Directions
1. Add 18.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into tubes or as desired.
