Appearance (Colour)
Light yellow to pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
31.50 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
5.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Pink
Cultural Response
Inoculate and incubate at 25-30°C for 2-7 days
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth : Good
Organism
Aspergillus niger ATCC 16404
Inoculum
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum
Growth : Inhibited
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation and enumeration of yeasts and moulds of significance in food spoilage.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
5.00
Dextrose
10.00
Monopotassium phosphate
1.00
Magnesium sulfate
0.5
Rose Bengal
0.025
Dichloran
0.002
Chloramphenicol
0.10
Agar
15.00
Directions
1. Add 31.50 gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Donot Over heat.
