Appearance (Colour)
Pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
56.68 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% agar gel
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 37°C for 24 hours
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red w/black centres
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red w/black centres
Organism
Salmonella paratyphi B
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red w/black centres
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Grey w/black centres
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : 30.0%
Colony colour : Red
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Fair
Recovery Rate : 30.0%
Colony colour : Yellow
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Yellow
Organism
Salmonella paratyphi A
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Yellow
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red w/black centres
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red
Organism
Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Red
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth : Inhibited
Applications
Used for the isolation and differentiation of enteric pathogens, especially Shigella and Providencia.
Composition
Ingredients
gm/lt.
Agar
15.00
Lactose
7.50
Sodium chloride
5.00
Sucrose
7.50
Sodium thiosulphate
6.80
L-Lysine
5.0
Xylose
3.50
Yeast extract
3.00
Sodium deoxycholate
2.50
Ferric ammonium citrate
0.80
Phenol Red
0.08
Directions
1. Add 56.68 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
4. Dispense into sterile petri plates.
