Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
41.53 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,Colony colour : Pink red w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,Colony colour : Pink
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,Colony colour : Colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>103, Growth : Inhibited
Organism
Klebsiella pneumoniae ATCC 13048
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%,Colony colour : Pink to pinkish red.
Applications
Recommended medium for selective isolation, detection and enumeration of coliforms in water, milk and other dairy products.
Composition
Ingredients
gm/lt.
Peptone
7.00
Yeast extract
3.00
Agar
15.00
Lactose
10.00
Sodium chloride
5.00
Bile salts
1.50
Neutral Red
0.03
Crystal Violet
0.002
Directions
1. Add 41.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave.
4. Dispense into sterile petri plates.
