Appearance (Colour)
White to cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
27.7 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 25°C - 30°C for upto 7 days.
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Micrcoccus luteus ATCC 10240
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Achromobacter denitrificans ATCC 14648
Inoculum (cfu) 10-100
Growth : Good
Applications
Recommended media for isolation and cultivation of denitrifying bacteria.
Composition
Ingredients
gm/lt.
L-Asparagine
1.00
Sodium citrate
8.50
Potassium nitrate
1.00
Potassium Dihydrogen Phosphate
1.00
Magnesium sulphate
1.00
Calcium chloride
0.20
Ferric chloride
0.0001
Agar
15.00
Directions
1. Add 27.7 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
