Appearance (Colour)
Cream
Appearance (Colour)
Free flowing, homogeneous powder
Solubility
40.25 gm/liter
Prepared Medium Appearance before autoclaving (Clarity)
Slightly opalescent to opalescent, with presence of slight precipitate.
pH (25°C)
7.6 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent to opalescent, with presence of slight precipitate. and few dark particles
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 25°C- 30°C for 40-72 hours
Organism
Vibrio fischeri ATCC 7744
Inoculum (cfu) 10-100
Growth : Good
Organism
Vibrio harvyi ATCC 14216
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for cultivation of heterotrophic marine bacteria.
Composition
Ingredients
gm/lt.
Peptone
5.00
Yeast extract
1.00
Ferric citrate
0.10
Sodium chloride
19.45
Magnesium chloride
8.80
Sodium sulphate
3.24
Calcium chloride
1.80
Potassium chloride
0.55
Sodium bicarbonate
0.16
Potassium bromide
0.08
Strontium chloride
0.034
Boric acid
0.022
Sodium silicate
0.004
Sodium fluorate
0.0024
Ammonium nitrate
0.0016
Disodium phosphate
0.008
Directions
1. Add 40.25 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense 50 ml of the broth in 250 ml Erlenmeyer flasks or as desired.
