Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
65.00 gm/liter
Solubility before autoclaving (Clarity)
Very slightly to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
6.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 25 ± 2°C for 18 -48 hours
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth : Good
Applications
For maintaining and propagating yeasts in molecular biology studies.
Composition
Ingredients
gm/lt.
Yeast extract
10.00
Dextrose
20.00
Peptone
20.00
Agar
15.00
Directions
1. Add 65.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
