Appearance (Colour)
Beige
Appearance (Form)
Free, flowing homogeneous powder
pH (25°C)
6.40 - 6.80
Solubility
15.8 gms/lit.
Solubility before autoclaving (Clarity)
Clear solution
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Prepared Medium Appearance after autoclaving (Clarity)
Clear solution without any precipitate
Cultural Response
Inoculate and incubate at 35 -37°C for upto 5 days.
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Spore formation : Positive
Organism
Bacillus pumilus ATCC 14884
Inoculum (cfu) 10-100
Growth : Good
Spore formation : Positive
Applications
Recommended medium for promoting sporulation in Bacillus subtilis.
Composition
Ingredients
gms/lt.
Peptic digest of animal tissue
6.00
Tryptone
4.00
Yeast extract
3.00
Beef extract (ex.buffalo)
1.50
Dextrose
1.00
Manganous sulphate
0.30
Directions
1. Add 15.8 gms powder to distilled/purified water and bring volume to 10 liter.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C fir 15 minutes.
