Appearance (Colour)
Clear solution
Solubility
1.0 %, Clear solution
Cultural Response
Cultural characteristics observed after inoculation with following organisms at 37°C for 24 hours on Lactose TTC Agar using TTC solution 1.0% (55052).
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Colony colour : red with blue corona
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good
Colony colour : red with blue corona
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Colony colour : orange yellow with yellow corona
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Colony colour : red with blue corona
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Colony colour : red with blue corona
Applications
Used for detecting microorganisms on the basis of TTC reduction.
Composition
2, 3, 5- Triphenyl Tetrazolium chloride
0.1 gm
Distilled water
10.0 ml
Directions
1. Aseptically,add to sterile cooled media at 45-50°C.
2. Mix well and pour into sterile petri plates.
Add following quantity in 1.0 liter media
2.5 ml :
Modified Tergitol-7 Agar Base
M Tergitol-7 Agar Base w/Meat Extract
Lactose TTC Agar (70637)
3 ml :
Tergitol 7 Agar Base
Tergitol 7 Broth
Tergitol 7 Agar H
Tryptone Sucrose Tetrazolium Agar base
5 ml :
Crystal Tetrazolium Agar Base (91022)
10 ml :
CAE Agar Base (16574)
KF Streptococcus Agar Base (64342)
KF Streptococcus Broth Base
KF Streptococcus Broth Base w/BCP
L.S. Differential Medium Base
M-Azide Broth
M-Slanetz Enterococcus Broth Base
Motility Medium S ,Base (90174)
Pagano levin Base (35973)
SM Selective Agar Base
MUD SF Broth Base
15 ml :
M-Enterococcus Agar Base,Modified
