Appearance (Colour)
Light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
96.02 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 37°C for 18 - 48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Fair - good
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation and cultivation of staphylococci.
Composition
Ingredients
gm/lt.
Proteose peptone
10.00
Sodium chloride
75.00
D-Mannitol
10.00
Beef extract (ex.buffalo)
1.00
Phenol Red
0.025
Directions
1. Add 96.02 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
