Appearance (Colour)
Beige with pinkish tinge
Appearance (Form)
Free flowing, homogeneous powder
Solubility
38.53 gm/liter
Solubility before boiling (clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.4 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to very slightly opalescent
Prepared medium appearance after boiling (colour)
Reddish purple
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Reecovery Rate : >=50.0%
Colony colour : Pink - red w/bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Reecovery Rate : >=50.0%
Colony colour : Pink - red
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Reecovery Rate : >=50.0%
Colony colour : Light pink
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Pink to purple
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Used for detection and enumeration of Enterobacteriaceae from foods.
Composition
Ingredients
gm/lt.
Peptone
7.00
Yeast extract
3.00
Agar
12.00
Glucose
10.00
Sodium chloride
5.00
Bile salts mixture no.3
1.50
Neutral Red
0.03
Crystal Violet
0.002
Directions
1. Add 38.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave. Do not overheat.
