Appearance (Colour)
Biege with slight green tint
Appearance (Form)
Free flowing, homogeneous powder
Solubility
36.14 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Green
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=70%,
Colony colour : Blue
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow to whitish blue
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth: Good, swarming inhibited, Recovery Rate:>=70%,
Colony colour : Blue to bluish green
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=70%,
Colony colour : Slight yellowish to greenish
Applications
For the isolation, enumeration and presumptive identification of microorganisms from urine.
Composition
Ingredients
gm/lt.
Peptone
4.00
Agar
15.00
Lactose
10.00
Tryptone
4.00
Beef extract (ex.buffalo)
3.00
L-Cystine
0.128
Bromothymol Blue
0.02
Directions
1. Add 36.14 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
