Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility (Colour) 2% aq. solution
Light to medium amber, clear without any precipitate
pH (25°C)
6.0 - 7.0 (2.0%)
Stability (After autoclaving)
Light to medium amber, clear without any precipitate
Loss on drying
< 5.0 %
Nitrogen content (N)
~ 12.0 -13.0%
Total Aerobic microbial count
(By Plate Count Method) : < 10,000 cfu/gm
Total Yeast and Mould count
(By Plate Count Method) : < 100 cfu/gm
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Clostridium perfringens
ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus
ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes
ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Indole Production (0.1%)
Positive
Fermentable carbohydrates (2%)
Negative
Hydrogen Sulphide Production (1.0%)
Positive
Residue on ignition
<= 12.0 %
Applications
Biopeptone is a mixture of enzymic digest of casein and meat peptone. It is recommended for the cultivation of fastidious microorganisms in Columbia Broth Base, Dextrose Agar and Casman Agar Base.