Appearance (Colour)
White to Off-white powder
Appearance (Form)
Free flowing, homogeneous powder
Solubility
20.00 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 2.0 % agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
White to Off-white
Cultural Response
Inoculate and incubate at 35 - 37° C for bateria and at 25°C for fungi for 48 -72 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Fair
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Fair
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth : Fair - Good
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Fair - Good
Applications
Used for cultivation and observation of sporulation of some fungi and also for the enumeration of fungi according to MPN method.
Composition
Ingredients
gm/lt.
Agar
20.00
Directions
1. Add 20.00 gm powder to 1.0 liter distilled/purified water.
2. Gently heat and bring to boiling.
3. Autoclave at 121°C at 15 psi pressure for 15 minutes.
