Appearance (Colour)
White to off white
Appearance (Form)
Free flowing homogeneous powder
Solubility
(1%) Soluble in distilled water, insoluble in alcohol & ether
Solubility before autoclaving (Clarity)
(1%) Clear and free from extraneous matter
pH (1% aq. solution)
7.00 - 9.00
Solubility after autoclaving (Clarity)
(1%) Clear and free from extraneous matter
Solubility after autoclaving (Colour)
1% aqueous solution is light straw coloured
Cultural Response: Inoculate and incubate at 37° C for 18-24hrs
Incubate at 37°C ± 2°C for 18-24 hours by preparing MacConkey Agar containing Bile salts mixture
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Pink to red with bile precipitate
Organism
Enterococcus aerogens ATCC 13048
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Pink to red
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Colourless
Organism
Salmonella paratyphi B ATCC 8759
Inoculum (cfu) 10-100
Growth- Good, Colony colour- Colourless
Applications
Used as an inhibitory agent for gram positive organisms in microbiological media.
