Appearance (Colour)
Reddish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
38.53 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.2% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 32 ± 2°C for 18-24 hours
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate :>=50.0%
Colony colour : pinkish red w/bile precipitate
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : red may have a slight red precipitate
Organism
Staphylococcus aureus
ATCC 25923
Inoculum
>=10³
Growth : Inhibited
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : colorless to beige colonies
Applications
Recommended medium for selective isolation, detection and enumeration of coliforms in water, milk and other dairy products.
Composition
Ingredients
gms/lt.
Peptone
7.00
Yeast extract
3.00
Lactose
10.00
Sodium chloride
5.00
Bile salts mixture
1.50
Neutral Red
0.03
Crystal Violet
0.002
Agar
12.00
Description
1. Add 38.53 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat with frequent agitation and bring to boiling.
3. Do not autoclave or overheat.
4. Cool to 45-50°C and dispense as desired.
