Appearance (Colour)
Creamish tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
30.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate (isolated colony) the tubes by stabbing the butt to the center through 2/3 rds of the medium with the following and incubate at 37°C for 18 -24 hours
After 24 hours of incubation observe the tubes for growth,motility,hydrogen sulphide (H2S) production and indole production by addition of 3 - 4 drops of Kovacs reagent
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)
Indole : (+)
H2S : (-)
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)
Indole : (-)
H2S : (+)
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Motility : (+)
Indole : (-)
H2S : (+)
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Motility : (-)
Indole : (-)
H2S : (-)
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Motility : (-)
Indole : (-)
H2S : (-)
Key
(+) Motiliy : is observed as diffused growth outward from the stab line or turbidity of the medium.
(+) H2S : is observed by blackening of the medium or along the stab line.
(+) Indole : is indiacted by a red colour after addition of Kovacs Reagent.
Applications
Used for the differentiation of members of the Enterobacteriaceae, based onH2S production, indole production and motility.
Composition
Ingredients
gm/lt.
Veg. Peptone
20.00
Veg. extract
6.71
Agar
3.00
Ferrous ammonium sulphate
0.20
Sodium thiosulphate
0.025
Directions
1. Add 30.00 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense the medium into test tubes to a depth of 3 inches preferably.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
