Appearance (Colour)
Red, crystalline powder
Solubility
Soluble in 0.2 N Sodium Hydroxide
Cultural Response
Inoculate and incubate at 35 ± 2°C and 45.5°C for 18-24 hours (Aseptically,add the rehydrated contents of 1 vial of Rosolic Acid Supplement (57672) as per direction)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth at 35 ± 2°C : Good
Growth at 45.5°C : Good
Colony colour : Light blue
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth at 35 ± 2°C : Good
Growth at 45.5°C : Inhibited
Colony colour : Pinkish
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth at 35 ± 2°C : Good
Growth at 45.5°C : Inhibited
Colony colour : Pinkish
Organism
Enterococcus faecalis ATCC 29212
Inoculum
Growth at 35 ± 2°C : Inhibited
Growth at 45.5°C : Inhibited
Applications
Used for the selective isolation of coliforms.
Composition
Rosolic Acid
0.10 gm/vial
Directions
1. Aseptically add 10 ml of sterile 0.2N Sodium Hydroxide to 1 vial.
2. Mix well and aseptically add to sterile cooled media at 45-50°C and dispense as desired.
3. Add 10 ml (1 vial) of Rosolic Acid Supplement to 990 ml of M-FC Agar Base (52295) or M-FC Broth Base (76439).
