Appearance (Colour)
Grey
Appearance (Form)
Free flowing, homogeneous powder
Solubility
62.50 gm/liter
Solubility before autoclaving (Clarity)
Opaque solution with precipitate
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opaque with precipitate
Prepared Medium Appearance after autoclaving (Colour)
Black
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours
Organism
Bordetella bronchiseptica ATCC 4617
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=50%
Organism
Bordetella pertussis ATCC 8467
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=50%
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103, Growth : Inhibited
Applications
For the cultivation and maintenance of fastidious microorganisms, especially Bordetella pertussis, for production of vaccines.
Composition
Ingredients
gm/lt.
Beef heart infusion from
500.00
Peptone
10.00
Yeast extract
3.50
Sodium chloride
5.00
Starch soluble
10.00
Charcoal
4.00
Agar
18.00
Directions
1. Add 62.50 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. If desired, add aseptically 10% of sterile defibrinated blood and rehydrated contents of 1 vial of Bordetella Selective Supplement (66006) to 500 ml of medium.
