Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
51.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red with purplish tinge
Cultural Response
Inoculate and incubate at 35 ± 2°C for 18-24 hours
Organism
Escherichia coli
ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Pink to red w/bile precipitate
Organism
Enterobacter aerogenes
ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Pink to red
Organism
Klebsiella pneumoniae
ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Pink to red, mucoid
Organism
Salmonella typhimurium
ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Colourless
Organism
Pseudomonas aeruginosa
ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Colony colour : Colourless
Organism
Proteus vulgaris
ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Colourless
Organism
Shigella flexneri
ATCC 12022
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery Rate : >=10.0%
Colony colour : Colourless
Organism
Proteus mirabilis
ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Colourless
Organism
Enterococcus faecalis
ATCC 29212
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery Rate : >=10.0%
Colony colour : pale pink- red
Organism
Staphylococcus aureus
ATCC 25923
Inoculum
>=10³
Growth : Inhibited
Applications
Selective medium for gram-negative organisms by suppressing the growth of a number of gram positive bacteria.
Composition
Ingredients
gms/lt.
Peptic digest of animal tissue
1.50
Pancreatic digest of gelatin
17.00
Enzymatic hydrolysate of casein
1.50
Agar
15.00
Lactose
10.00
Bile salts
1.50
Sodium chloride
5.00
Crystal Violet
0.001
Neutral Red
0.03
Directions
1. Add 51.50 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
