Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
32.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 37°C for 24 - 48 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=70%
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=70%
Applications
To determine the phenol coefficient of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus ATCC 6538.
Composition
Ingredients
gm/lt.
Enzymatic hydrolysate of casein
5.00
Beef extract
3.00
Polysorbate 80
7.00
Lecithin
1.00
Dextrose
1.00
Agar
15.00
Directions
1. Add 32.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
