Appearance (Colour)
Mustard yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
24.28 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
pH (25°C)
6.9 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Forest green
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Medium colour : Blue
Citrate utilization : (+ve)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Medium colour : Blue
Citrate utilization : (+ve)
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Medium colour : Blue
Citrate utilization : (+ve)
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Fair-good
Medium colour : Green
Citrate utilization : (-ve)
Organism
Escherichia coli ATCC 25922
Inoculum
>=103,Growth : Inhibited
Organism
Shigella dysenteriae ATCC 13313
Inoculum
>=103,Growth : Inhibited
Applications
For the differentiation of gram-negative bacteria on the basis of citrate utilization.
Composition
Ingredients
gm/lt.
Agar
15.00
Sodium chloride
5.00
Sodium citrate
2.00
K2HPO4
1.00
(NH4)H2PO4
1.00
MgSO4.H2O
0.20
Bromothymol Blue
0.08
Directions
1. Add 24.28 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
