Appearance (Colour)
Light pink
Appearance (Form)
Free flowing, homogeneous powder
Solubility
111.025 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 37°C for 18- 48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : Yellow
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : Yellow
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Fair-good
Recovery rate : >=40.0%
Colony colour : Red
Organism
Escherichia coli ATCC 25922
Inoculum
Growth : Inhibited
Organism
Enterobacter aerogenes ATCC 13048
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation, cultivation and enumeration of staphylococci from clinical and non-clinical specimens.
Composition
Ingredients
gm/lt.
Agar
15.00
Peptone
5.00
Sodium chloride
75.00
D-Mannitol
10.00
Enzymatic digest of casein
5.00
Beef extract (ex.buffalo)
1.00
Phenol Red
0.025
Directions
1. Add 111.025 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
