Appearance (Colour)
White
Appearance (Form)
Crystalline powder
Solubility (Turbidity) 1% aq. solution
Clear to slight opalascent
Activity
min. 7500 BAEE units/mg
min. 2500 NF units/mg
Unit Definition
One BAEE unit of Trypsin activity will produce ΔA at 253nm of 0.001 per minute with BAEE as substrate at pH 8.0 at 25 °C in a reaction volume of 3.0 mL.
3 BAEE units = 1 NF unit
Enzyme solution for activity determination
Prepared in 0.001 N Tris HCl.
