Appearance (Colour)
Blue
Appearance (Form)
Powder
Absorbance @244 nm (0.6mg/mL in 20mM HEPES Buffer @ pH 7.2)
0.2-0.3
Microbiological and Biological assays (antibiogram on bacteria reference strain)
80-120%
Cytotoxicity on resistant mammalian cell lines
> 960 μg/mL
Cytotoxicity on sensitive mammalian cell lines
≤ 120 μg/mL
Residual Cytotoxicity Long term, on mammalian cell lines
Undetectable after 4 pasages
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg)
< 1.0 EU/mg
Applications
Phleomycin is a glycopeptide antibiotic of the bleomycin family that exhibits in vivo activity against bacteria, eukaryotic microorganisms, plant and animal cells.