Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
15.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Indole production : (+)
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Good
Indole production : (+)
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Indole production : (-)
Key
Indole (+) red colour at the interface of medium
Indole (-) no colour at the interface of medium
Applications
For the cultivation of production of Indole by microorganisms.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
10.00
Sodium chloride
5.00
Directions
1. Add 15.00 gm powder to distilled/purified water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Dispense into test tubes.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
