Stability
The competent cells should be viable for three to six months from the date of manufacture when stored as instructed.
Activity
All components of kit were tested for transformation of E.coli strain with supercoiled plasmid pUC19
Description
Transformation, a process central to recombinant DNA technology, is the process by which a foreign DNA is introduced into a bacterium. In this kit, the experiment employs a 3-5kb circular Plasmid DNA as the foreign DNA. The Plasmid contains genes of interest to us along with a selection markers (i.e., genes which confer antibiotic resistance). For Transformation to be successful, the recipient bacterium, called the Host, should first be made Competent for the uptake of DNA. This kit describes a simple procedure for the same. The competent status, however, is not permanent and is lost eventually. In general, freshly made competent cells provide high Transformation efficiency.
Includes
| Components | 15 Experiments | 30 Experiments |
|---|
| Host cells | 1.2 ml | 2.0 ml |
| Sterile LB medium | 50.0 ml | 60.0 ml |
| Sterile LB medium | 10.0 ml | 15.0 ml |
| Competent cells | 3.0 ml | 5.0 ml |
| Plasmid DNA (50 ng/mcl) | 40.0 mcl | 80.0 mcl |
| 0.1M CaCl2 | 30.0 ml | 60.0 ml |
| LB Agar | 18.0 gm | 36.0 gm |
| Ampicillin | 400 mg | 800 mg |
| Sterile water | 1.0 ml | 2.0 ml |
