Appearance (Colour)
Brownish yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
43.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 3.0% Agar gel
pH (25°C)
6.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate at 25-30°C for 3-6 days
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good, with absence of ascospore
Recovery Rate : >=50.0%
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth : Good, with presence of ascospore
Recovery Rate : >=50.0%
Applications
Ascospore Agar is a solid medium recommended for use in qualitative procedures for the cultivation of ascomycetous yeasts.
Composition
Ingredients
gm/lt.
Yeast extract
2.50
Dextrose
1.00
Potassium acetate
10.00
Agar
30.00
Directions
1. Add 43.50 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into sterile test tubes or petri plates.
