Appearance (Colour)
White to off white
Appearance (Form)
Crystalline powder
Activity
min. 200 units/mg
Unit Definition
One unit will liberate 1.0 micromole of NH3 from urea per minute at pH 7.0 at 25°C.
Enzyme solution for activity determination
Prepared in 10 mM potassium phosphate buffer (with 10 mM lithium chloride and 1 mM EDTA), pH 8.2.
Application
Applications:
Urease from Jack bean has been used to determine the hydrolysis rate of urea in pure urine. It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.
